PI15 (aa128-141) Antibody

SKU: orb12386

Description

Goat polyclonal to PI15.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:1000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 33kDa band observed in lysates of cell line HeLa (calculated MW of 29.1kDa according to NP_056970.1). Recommended concentration: 0.3-1µg/ml. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.. IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PI15 (aa128-141)
• Molecular Weight: 29.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PI15 antibody

orb12386 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb12386 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PI15 (aa128-141)

NCBI Gene ID

51050