Goat anti-PAR6alpha / PARD6A Antibody

SKU: orb18626

Description

Goat polyclonal antibody to PARD6A

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 5-10 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration, 10ug/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverWB: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PAR6alpha / PARD6A
• Molecular Weight: 37.4; 37.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PARD6A antibody, anti PAR6alpha antibody, anti PAR-6 antibody, anti TAX40 antibody, anti PAR-6A antibody, anti TIP-40 antibody, anti par-6 partitioning defective 6 homolog alpha (C.elegans) antibody, anti Tax interaction protein 40 antibody, anti par-6 (partitioning defective 6, C.elegans) homolog alpha antibody, anti PAR6 antibody, anti PAR6C antibody, anti Tax-interacting protein 40 antibody, anti par-6 partitioning defective 6 homolog alpha antibody, anti partitioning defective-6 homolog alpha antibody, anti partitioning-defective protein 6 antibody

orb18626 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb18626 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of Jurkat (Lane 1) and U251 (Lane 2) cell lysate using PARD6A antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using PARD6A antibody

Quick Database Links

Gene Symbol

PAR6alpha / PARD6A

NCBI Gene ID

50855