Goat anti-NOS1 Antibody

• Catalog Number: orb18337
• Category: Antibodies
• Description: Goat polyclonal antibody to NOS1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, IHC-P: 2.5μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 161; 164.6; 125
• Target: NOS1
• Entrez: 4842
• Protein Sequence: ESKKDTDEVFSS
• RRID: AB_10754443
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NOS1 antibody, anti nitric oxide synthase 1 (
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: This product has been sucessfully used for IHC on Olfactory bulb in mice (PMID: 20140458)WB: Approx 160-170kDa band observed in Human Skeletal Muscle and Mouse Brain lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.3-1 μg/ml.Experiment Notes: Immunofluorescence: This product has been successfully used for IF as reported (PMID: 20140458).
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of mouse caudate-putamen using NOS1 antibody

Flow cytometric analysis of Kelly cells using NOS1 antibody

Immunofluorescence analysis of HeLa cells using NOS1 antibody

Immunohistochemical staining of Human Cortex using NOS1 antibody

1 µg/mL staining of Mouse Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunostaining of 30 µm thick cryosections of PFA-perfused Human Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.