Goat anti-NMNAT3 (aa149-162) Antibody

• Catalog Number: orb125179
• Category: Antibodies
• Description: Goat polyclonal antibody to NMNAT3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 24.1; 18.3
• Target: NMNAT3 (aa149-162)
• Entrez: 349565
• Protein Sequence: QHNIHLAKEPVQNE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NMNAT3 antibody, anti nicotinamide nucleotide
• Note: For research use only
• Application notes: WB: Approx 26kDa band observed in Human Kidney lysates (calculated MW of 24.1kDa according to NP_835471.1). Recommended concentration: 0.1-0.3µg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.