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Catalog Number | orb19035 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to NANOS1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:64000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 30.2 |
Target | NANOS1 |
Entrez | 340719 |
Protein Sequence | SARDGPPGKKLR |
RRID | AB_10767257 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti NANOS1 antibody, anti nanos homolog 1 (Drosop Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1;13(7):727-39. Epub 2004 Feb 12, PMID: 14962983). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19035 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.