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Catalog Number | orb18315 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ITK |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Human, Mouse |
Dilution range | ELISA: 1:2000, WB: 1-3g/ml, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 71.8 |
Target | ITK |
Entrez | 3702 |
Protein Sequence | RLLRQLAEIAESGL |
RRID | AB_10749738 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ITK antibody, anti EMT antibody, anti LYK ant Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Jurkat nuclear cell lysate (A) and negative control Human parathyroid gland (B) using ITK antibody.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ITK antibody.
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells using ITK antibody.
ELISA, FC, IF, IHC, WB | |
Bovine, Human, Rat | |
Goat | |
Polyclonal | |
Unconjugated |