Goat anti-ITGAM / CD11B Antibody

• Catalog Number: orb19554
• Category: Antibodies
• Description: Goat polyclonal antibody to CD11b
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:32000, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 127
• Target: ITGAM / CD11B
• Entrez: 3684
• Protein Sequence: RNDGEDSYRTQ
• RRID: AB_10749632
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Antigen CD11b p170 antibody, anti CD11 antige
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 127kDa according to NP_000623.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19554 (2µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Immunofluorescence analysis of U937 cells using CD11b antibody.

Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

8 µg/mL staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).