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Catalog Number | orb334083 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody against GAPDH conjugated to Biotin. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Mouse, Porcine |
Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 36.1; 31.5 |
Target | GAPDH (C Terminus) Loading Control, biotinylated |
Entrez | 2597 |
Protein Sequence | HQVVSSDFNSDT. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | Anti-GAPDH antibody, Anti- glyceraldehyde-3-phosph Read more... |
Note | For research use only |
Application notes | WB: Approx 35kDa band observed in lysates of cel line HEK293 (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293 lysate using GAPDH antibody (Biotin)