Goat anti-GAPDH (C Terminus) Loading Control Antibody

• Catalog Number: orb18774
• Category: Antibodies
• Description: Goat polyclonal antibody to GAPDH
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine
• Dilution range: ELISA: 1:128000, WB: 0.001-0.003 μg/ml, IHC-P: 2.5 μg/ml, IF/ICC: 10μg/ml
• Conjugation: Unconjugated
• MW: 36.1
• Target: GAPDH (C Terminus) Loading Control
• Entrez: 2597
• Protein Sequence: HQVVSSDFNSDT
• RRID: AB_10750737
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GAPDH antibody, anti glyceraldehyde-3-phospha
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

0.1 µg/ml staining of HEK293 (A), HeLa (B) and A549 (C) and 0.01 ug/ml LNCaP (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

0.1 µg/ml staining of Human Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

4 µg/ml staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

5 µg/ml staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.