Goat anti-Ezrin / villin 2 Antibody

• Catalog Number: orb19239
• Category: Antibodies
• Description: Goat polyclonal antibody to VIL2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 69.4
• Target: Ezrin / villin 2
• Entrez: 7430
• Protein Sequence: QDYEEKTKKAER
• RRID: AB_10750668
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti villin 2 (ezrin) antibody, anti ezrin antibod
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Small Intestine shows strong staining of enterocytes. Recommended concentration, 3-5ug/ml.WB: Approx 80kDa band observed in Human Duodenum and Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Placenta lysate using VIL2 antibody

Immunohistochemical staining of paraffin embedded Human Small Intestine using VIL2 antibody

Immunohistochemical staining of Human Kidney using VIL2 antibody