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Catalog Number | orb19738 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to EXO1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:64000, WB: 0.2-0.5 μg/ml |
Conjugation | Unconjugated |
MW | 89.1; 94.0 |
Target | EXO1 |
Entrez | 9156 |
Protein Sequence | HRNYSPRPESGT |
RRID | AB_10753581 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti EXO1 antibody, anti exonuclease 1 antibody, a Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3). Recommended concentration: 0.2-0.5 μg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
orb19738 (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.