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Item 1 of 5
Goat anti-DKK1 Antibody
Catalog Number: orb18888
| Catalog Number | orb18888 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to DKK1 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Bovine, Human, Mouse |
| Concentration | 10 μg/ml |
| Dilution range | ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml |
| Conjugation | Unconjugated |
| MW | 28.7 |
| Target | DKK1 |
| Entrez | 22943 |
| Protein Sequence | CDHHQASNSSRLHT |
| RRID | AB_10755733 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti DKK1 antibody, anti SK antibody, anti DKK-1 a Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa band observed in human spleen and bone marrow lysates after 1 μg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463). However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
orb18888 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
orb18888 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
orb18888 (10µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb18888 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Western blot analysis of Human Bone Marrow lysate using DKK1 antibody
