Goat anti-DDX5 / p68 RNA helicase Antibody

• Catalog Number: orb18641
• Category: Antibodies
• Description: Goat polyclonal antibody to DDX5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Concentration: 0.3-1 μg/ml
• Dilution range: ELISA: 1:2000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 69.1
• Target: DDX5 / p68 RNA helicase
• Entrez: 1655
• Protein Sequence: PMIGYPMPTGYSQ
• RRID: AB_10754603
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DDX5 antibody, anti P68 antibody, anti HLR1 a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1kDa according to NP_004387). Recommended concentration: 0.11-0.3 μg/ml.Experiment Notes: Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A431 cell lysate using DDX5 antibody

Immunohistochemical staining of Human Prostate using DDX5 antibody

Western blot analysis of NIH3T3 (Lane 1), HeLa (Lane 2), A431 (Lane 3) and Jurkat (Lane 4) using DDX5 antibody

Immunohistochemical staining of Human Uterus using DDX5 antibody

Immunohistochemical staining of Human Breast using DDX5 antibody