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Catalog Number | orb19207 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CYP17A1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Canine, Feline, Human |
Dilution range | ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml |
Conjugation | Unconjugated |
MW | 57.4 |
Target | CYP17A1 |
Entrez | 1586 |
Protein Sequence | LFKDGDQKLEK |
RRID | AB_10755908 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti CYP17A1 antibody, anti cytochrome P450, famil Read more... |
Note | For research use only |
Application notes | IHC: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration, 3-5μg/ml.WB: Approx 60kDa band observed in human ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
CYP17A1 antibody (0.3 µg/ml) staining of human ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical analysis of formalin-fixed and paraffin embedded human adrenal gland tissue using CYP17A1 antibody