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Catalog Number | orb19519 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CXCR3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC |
Reactivity | Human, Mouse, Rat |
Dilution range | ELISA: 1:64000, WB: 1 μg/ml, IHC-P: 10ug/ml |
Conjugation | Unconjugated |
MW | 40.7; 45.5 |
Target | CXCR3 / GPR9 |
Entrez | 2833 |
Protein Sequence | RRDSSWSETSEA |
RRID | AB_10749218 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti CXCR3 antibody, anti chemokine (C-X-C motif) Read more... |
Note | For research use only |
Application notes | WB: Preliminary experiments showed a band at approx 55-60kDa in Human Tonsil and Mouse Spleen lysates after 1µg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 6-8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration |
Expiration Date | 12 months from date of receipt. |