Goat anti-Chat (mouse) Antibody

• Catalog Number: orb19261
• Category: Antibodies
• Description: Goat polyclonal antibody to Chat
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 1:2000
• Conjugation: Unconjugated
• MW: 72.3
• Target: Chat (mouse)
• Protein Sequence: KERARGPTKPSNLD
• RRID: AB_10755556
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-Acetyl CoA choline O acetyltransferase antibo
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75-80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19261 (0.2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Mouse Brain lysate using Chat antibody