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Catalog Number | orb18994 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to BID |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Human |
Dilution range | ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 26.8; 22.0; 11.3 |
Target | BID |
Entrez | 637 |
Protein Sequence | TYVRSLARNGMD |
RRID | AB_10755547 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti Apoptic death agonist antibody, anti BH3 inte Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 26+24kDa bands observed in Human fibroblast A431 lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1). Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb18994 (0.1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence analysis of MCF7 cells using BID antibody