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Item 1 of 2
Goat anti-Aurora kinase A / AURKA Antibody
Catalog Number: orb18316
| Catalog Number | orb18316 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to AURKA |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Human |
| Dilution range | ELISA: 1:128000, WB: 0.1-0.3 μg/ml |
| Conjugation | Unconjugated |
| MW | 45.8 |
| Target | Aurora kinase A / AURKA |
| Entrez | 6790 |
| Protein Sequence | QNKESASKQS |
| RRID | AB_10746535 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti AURKA antibody, anti aurora kinase A antibody Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
orb18316 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with orb18316 in the right panel (vector-only transfection in first and fourth lanes).
