Goat anti-ALS2CR1 / NIF3L1 Antibody

• Catalog Number: orb18452
• Category: Antibodies
• Description: Goat polyclonal to ALS2CR1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:32000, WB: 0.2-1 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 42.0; 39.0
• Target: ALS2CR1 / NIF3L1
• Entrez: 60491
• Protein Sequence: ILSETDRDPLQVV
• RRID: AB_10767013
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NIF3L1 antibody, anti ALS2CR1 antibody, anti
• Note: For research use only
• Application notes: This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells. Recommended concentration, 2-4ug/ml.WB: Approx 40kDa band seen in Human Heart and Human Testis extracts [Predicted MW of approx. 39kDa according to NP_068596]. Recommended for use at 0.2-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18452 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18452 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.