Goat ACTH ELISA Kit

• Catalog Number: orb781058
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Adrenocorticotropic Hormone(ACTH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adrenocorticotropic Hormone(ACTH).
• Tested applications: ELISA
• Reactivity: Goat
• Range: 31.25-2000 pg/mL
• Concentration: 2000 pg/mL
• Sensitivity: 8.29 pg/mL
• Protein Length: 2h
• Alternative names: Corticotropin ELISA Kit, Adrenocorticotropin ELISA
• Note: For research use only
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat ACTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat ACTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat ACTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat ACTH in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.