GNB3 Antibody

• Catalog Number: orb1249017
• Category: Antibodies
• Description: GNB3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: SGHDNRVSCLGVT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2kDa according to NP_002066.1. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GNB3
• UniProt ID: P16520
• NCBI: NP_002066.1, NP_067642.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GNB3, guanine nucleotide binding protein (G protei
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2kDa according to NP_002066.1. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249017 (0.1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249017 (3.8 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.