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Catalog Number | orb344646 |
---|---|
Category | Antibodies |
Description | Glypican-1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-Glypican-1 protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein. |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 10µg/mL, WB: 1:500- 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Glypican-1 was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | P35052 |
NCBI | 30410985 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Glypican-1 Antibody, Glypican 1, Glypi Read more... |
Note | For research use only |
Application notes | Anti-Glypican-1 antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kDa in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kDa) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Rabbit anti-Glypican antibody. Tissue: human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Glypican antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50 ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5000 at 4°C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61 kDa, 61 kDa for Glypican-1. Other band(s): 110 kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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