Glycophorin A Antibody

• Catalog Number: orb749548
• Category: Antibodies
• Description: Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GYPA/280
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Glycophorin A antibody is available for research use only.
• UniProt ID: P02724
• Storage: Store the Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Glycophorin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC-P: Formalin-fixed, paraffin-embedded human angiosarcoma stained with Glycophorin A antibody (clone GYPA/280).

IHC-P: Formalin-fixed, paraffin-embedded human angiosarcoma stained with Glycophorin A antibody (clone GYPA/280).

IHC-P: Formalin-fixed, paraffin-embedded human placenta stained with Glycophorin A antibody (clone GYPA/280).

Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone GYPA/280). Expected molecular weight: routinely observed at ~16/38 kDa.

SDS-PAGE analysis of purified, BSA-free Glycophorin A antibody (clone GYPA/280) as confirmation of integrity and purity.