Glycophorin A Antibody

• Catalog Number: orb606431
• Category: Antibodies
• Description: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rGYPA/280
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human Glycophorin A protein was used as the immunogen for the recombinant GYPA antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant GYPA antibody is available for research use only.
• UniProt ID: P02724
• Storage: Store the recombinant GYPA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant GYPA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human angiosarcoma with recombinant GYPA antibody (clone rGYPA/280). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone rGYPA/280). Expected molecular weight: routinely observed at ~16/38 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant GYPA antibody (clone rGYPA/280) as confirmation of integrity and purity.