Glycogen Synthase 1 (phospho-S641) antibody

• Catalog Number: orb345405
• Category: Antibodies
• Description: Glycogen Synthase 1 (phospho-S641) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a S641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS641 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P13807
• NCBI: AAH03182.1
• Storage: Store Phospho GYS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-muscle glycogen synthase pS641 antibod
• Note: For research use only
• Application notes: This phospho specific polyclonal antibody was tested by immunoblotting, immunohistochemistry, and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunoprecipitation.
• Expiration Date: 12 months from date of receipt.

Affinity Purified Phospho-specific pS641 antibody to human muscle Glycogen Synthase (GS). Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'. Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'. Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'. Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4 nM for 45'. Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'. Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'. Load: 12 µL. Primary Antibody: pS641 at 1:1000. Secondary Antibody: HRP conjugated Gt-a-Rabbit IgG (orb347654) at 1:5000 dilution. Other detection methods will yield similar results.

Diagram of glycogen synthase as a component of insulin signal transduction pathways.

Immunohistochemistry with Anti-Glycogen Synthase antibody. Tissue: Human Prostate. Fixation: formalin-fixed, paraffin-embedded tissue. Antigen retrieval: heat-induced. Primary antibody: 5 µg/ml. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.