GLUT3 Rabbit Polyclonal Antibody

• Catalog Number: orb10727
• Category: Antibodies
• Description: GLUT3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT3 (151-260/493aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: SLC2A3
• UniProt ID: P11169
• RRID: AB_10752502
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: facilitated glucose transporter member 3; Glucose
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jones, Carolyn J. P. et al. Functional changes in Hofbauer cell glycobiology during human pregnancy Placenta, 36, 1130-1137 (2015)

PubMed: 26264896

Applications

IHC

Reactivity

Human

Sample: 293T (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 63 kD.

Sample: Brain (Mouse) Lysate at 40 ug, Brain (Rat) Lysate at 40 ug, 293T (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) CellLysate at 30 ug, Primary: Anti-GLUT3 (orb10727) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 50/63 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated (orb10727) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.