GLUT1 Antibody / SLC2A1

• Catalog Number: orb606925
• Category: Antibodies
• Description: Recognizes a protein of 45-55kDa, which is identified as GLUT1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut1 to Glut12. Glut1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut1 is expressed at variable levels in many human tissues. Overexpression has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: GLUT1/3132R
• Tested applications: ELISA, FACS, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 203-305 from the human protein was used as the immunogen for this recombinant GLUT1 antibody.
• Dilution range: ELISA: 1-2ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant GLUT1 antibody is available for research use only.
• UniProt ID: P11166
• Storage: Store the recombinant GLUT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant GLUT1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tongue with recombinant GLUT1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of human K562 cells with recombinant GLUT1 antibody (clone GLUT1/3132R); Red=isotype control, Blue= recombinant GLUT1 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant GLUT1 antibody as confirmation of integrity and purity.