Glucose Oxidase antibody

• Catalog Number: orb344169
• Category: Antibodies
• Description: Glucose Oxidase antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Fungi
• Isotype: IgG
• Immunogen: Glucose Oxidase [Aspergillus niger]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: User Optimized, WB: 1:1000 - 1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Glucose Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P13006
• NCBI: P13006.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Glucose Oxidase Antibody, Beta D Glucose
• Note: For research use only
• Application notes: Anti-Glucose Oxidase has been tested in western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Biorbyt Goat anti Glucose Oxidase antibody was used to detect Glucose Oxidase under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biorbyt Goat anti-Glucose Oxidase antibody at 1:5K in orb348637, ON 4°C. Primary antibody was detected with Biorbyt Dylight 649 conjugated Donkey anti-Goat at 1:10K 1.5 hr RT in orb348637.

Western Blot of Goat anti-Glucose Oxidase antibody. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Glucose Oxidase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody (p/n orb346990) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65-70 kDa, 65-70 kDa for Glucose Oxidase. Other band(s): None.