Glucose Oxidase antibody

• Catalog Number: orb344168
• Category: Antibodies
• Description: Glucose Oxidase antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Fungi
• Isotype: IgG
• Immunogen: Glucose Oxidase [Aspergillus niger]
• Concentration: 1 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: User Optimized, WB: 1:1000 - 1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Glucose Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P13006
• NCBI: P13006.1
• Storage: Store Glucose Oxidase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Glucose Oxidase Antibody, Beta D Glucose
• Note: For research use only
• Application notes: Anti-Glucose Oxidase has been tested in western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Biorbyt Goat anti Glucose Oxidase antibody was used to detect Glucose Oxidase under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biorbyt Goat anti-Glucose Oxidase antibody at 1:5K in orb348637, ON 4°C. Primary antibody was detected with Biorbyt Dylight 649 conjugated Donkey anti-Goat at 1:10K 1.5 hr RT in orb348637.

Western Blot of Goat anti-Glucose Oxidase antibody. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Glucose Oxidase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody (p/n orb346990) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65-70 kDa, 65-70 kDa for Glucose Oxidase. Other band(s): None.