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Catalog Number | orb344932 |
---|---|
Category | Antibodies |
Description | Glucose Oxidase antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Fungi |
Isotype | IgG |
Immunogen | Glucose Oxidase [A.niger] |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: User Optimized, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.niger]. Cross reactivity against Glucose Oxidase from other sources may occur but have not been specifically determined. |
Conjugation | Biotin |
UniProt ID | P13006 |
NCBI | P13006.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Glucose Oxidase Antibody biotin Conjug Read more... |
Note | For research use only |
Application notes | Anti-GLUCOSE OXIDASE Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Glucose Oxidase antibody Biotin Conjugated. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Glucose Oxidase antibody Biotin conjugated at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for Glucose Oxidase.
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Algae | |
Rabbit | |
Polyclonal | |
Biotin |
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