Glucocorticoid Receptor/NR3C1 Antibody

• Catalog Number: orb251525
• Category: Antibodies
• Description: Glucocorticoid Receptor/NR3C1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NR3C1 recombinant protein (Position: A20-F199). Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 85659 MW
• UniProt ID: P04150
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glucocorticoid receptor;GR;Nuclear receptor subfam
• Note: For research use only
• Application notes: WB: The detection limit for NR3C1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of NR3C1 using anti-NR3C1 antibody.Lane 1:human placenta tissue; 2:human HeLa cell; 3:rat brain tissue; 4:rat liver tissue; 5:mouse brain tissue;6:mouse liver tissue.

IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue.

IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in a paraffin-embedded section of rat cardiac muscle tissue.