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Catalog Number | orb344208 |
---|---|
Category | Antibodies |
Description | Glucoamylase antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Other |
Isotype | IgG |
Immunogen | Glucoamylase [Rhizopus] |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000 |
Form/Appearance | Lyophilized |
Purity | Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]. Cross reactivity against Glucoamylase from other sources is unknown. |
Conjugation | Unconjugated |
UniProt ID | P07683 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Glucoamylase Antibody, 1 4 alpha D gluca Read more... |
Note | For research use only |
Application notes | Anti-Glucoamylase Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Glucoamylase [Rhizopus] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti Glucoamylase antibody (orb750407) was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).
Western Blot of Goat Anti-Glucoamylase Antibody. Lane 1: Molecular Weight Marker. Lane 2: detection of Anti-Glucoamylase Antibody.
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