GLS2 Antibody

• Catalog Number: orb1239416
• Category: Antibodies
• Description: GLS2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.The immunogen is located within amino acids 300 - 350 of GLS2.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa
• Target: GLS2
• UniProt ID: Q9UI32
• NCBI: NP_037399
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase
• Note: For research use only
• Application notes: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Validation of GLS2 in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling GLS2 with orb1239416 at 20 µg g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Immunohistochemistry Validation of GLS2 in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLS2 antibody (orb1239416) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of GLS2 in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GLS2 antibody (orb1239416) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of GLS2 in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GLS2 antibody (orb1239416) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

WB Validation in Human Pancreas. Loading: 10 µg of lysate Antibodies: GLS2, orb1239416, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse and Rat Brain. Loading: 15 µg of lysate Antibodies: GLS2, orb1239416, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.