GLS Antibody (C-term)

• Catalog Number: orb1788024
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 516-545 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 73461 Da
• Target: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
• UniProt ID: O94925
• NCBI: NP_001243239.1, NP_055720.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Glutaminase kidney isoform, mitochondrial, GLS, K-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

All lanes: Anti-GLS Antibody (C-term) at 1:1000 dilution. Lane 1: mouse brain lysates. Lane 2: human brain lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 73 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with XAF1 GLS Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).