glpK antibody

• Catalog Number: orb750399
• Category: Antibodies
• Description: glpK antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Isotype: Antiserum
• Immunogen: Glycerol Kinase [Cellulomonas species]
• Concentration: 90 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glycerol Kinase [Cellulomonas species]. Cross reactivity against Glycerol Kinase from other sources is unknown.
• Conjugation: Unconjugated
• UniProt ID: D0VZG4
• NCBI: BAI49417.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Glycerol Kinase Antibody, ATP glycerol 3
• Note: For research use only
• Application notes: Anti-Glycerol Kinase has been tested by western blot and is suitable to be assayed against 1.0 µg of Glycerol Kinase [Celullomonas species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Biorbyt Goat anti Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).

Both the antiserum and IgG fractions of anti-Glycerol Kinase (Cellulomonas) are shown to detect the 128000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:4000 dilution of the primary antibody is used followed by detection using HRP Rabbit-a-Goat IgG [H&L] (orb346990) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.