GLO1 Antibody / Glyoxalase I

• Catalog Number: orb639684
• Category: Antibodies
• Description: GLO1 is an enzyme involved in the detoxification of methylgyoxal, a byproduct of glycolysis. GLO1 expression has been demonstrated by several studies to be upregulated in various human malignant tumors, including metastatic melanoma and lung carcinoma, and thus is a target for pharmaceutical development.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-GLO1-3
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length human recombinant protein was used as the immunogen for the Glyoxalase 1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Glyoxalase 1 antibody is available for research use only.
• UniProt ID: Q04760
• Storage: Store the Glyoxalase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human thyroid carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human prostate carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HeLa and HepG2 cell lysate with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Predicted molecular weight ~21 kDa.

SDS-PAGE analysis of purified, BSA-free Glyoxalase 1 antibody (clone CPTC-GLO1-3) as confirmation of integrity and purity.