GLO1 Antibody / Glyoxalase I

• Catalog Number: orb606894
• Category: Antibodies
• Description: GLO1 is an enzyme involved in the detoxification of methylgyoxal, a byproduct of glycolysis. GLO1 expression has been demonstrated by several studies to be upregulated in various human malignant tumors, including metastatic melanoma and lung carcinoma, and thus is a target for pharmaceutical development.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-GLO1-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length human protein was used as the immunogen for the GLO1 antibody.
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This GLO1 antibody is available for research use only.
• UniProt ID: Q04760
• Storage: Store the GLO1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the GLO1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma stained with GLO1 antibody. Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using GLO1 antibody. These results demonstrate the foremost specificity of the CPTC-GLO1-1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human prostate carcinoma stained with GLO1 antibody. Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human thyroid carcinoma stained with GLO1 antibody. Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HeLa cell lysate with GLO1 antibody. Predicted molecular weight ~21 kDa.