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Catalog Number | orb1231213 |
---|---|
Category | Proteins |
Description | GITR / TNFRSF18 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Mouse IgG Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 41.4 kDa |
Target | TNFRSF18 |
UniProt ID | Q9Y5U5 |
Protein Sequence | Gln 26 - Glu 161 |
Source | HEK293 cells |
NCBI | NP_004186.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | AITR,GITR,TNFRSF18,CD357 Read more... |
Note | For research use only |
Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-49 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human GITR, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human GITR Ligand, Fc Tag at 5 ug/ml (100 uL/well) can bind Human GITR, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.039-0.313 ug/ml.
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
41.0 kDa | |
HEK293 cells |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
15.8 kDa | |
HEK293 cells |