GFRAL Antibody (C-term)

• Catalog Number: orb1938483
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28881
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 44518 Da
• Target: This GFRAL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the C-terminal region of human GFRAL.
• UniProt ID: Q6UXV0
• NCBI: NP_997293.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GDNF family receptor alpha-like, GFRAL, C6orf144
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-GFRAL Antibody (C-term) at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: MDA-MB-453 whole cell lysate. Lane 4: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-GFRAL Antibody (C-term) at 1:1000 dilution + Human brain whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

GFRAL Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control (Rabbit IgG isotype) (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

GFRAL antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFRAL antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.