Gfra3 Antibody

• Catalog Number: orb1238921
• Category: Antibodies
• Description: Gfra3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: GFR alpha 3 antibody was raised against a 14 amino acid peptide near the center of murine GFR alpha 3.The immunogen is located within the last 50 amino acids of GFR alpha 3.
• Concentration: 1 mg/ml
• Dilution range: GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 44 kDa Observed: 43 kDa
• Target: Gfra3
• UniProt ID: O35118
• NCBI: NP_034410
• Storage: GFR alpha 3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: GFR alpha 3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: GFR alpha 3 Antibody: Y15110, GFRalpha3, GDNF fami
• Note: For research use only
• Application notes: GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 µg/mL.

Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 µg/mL.

Immunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 µg/ml. Green: GFR alpha 3 antibody (orb1238921) Red: Phylloidin staining Blue: DAPI staining.

Immunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 µg/ml.