GFP Western Blot Kit antibody

• Catalog Number: orb345938
• Category: Assays and Kits
• Description: GFP Western Blot Kit antibody
• Clonality: Monoclonal
• Tested applications: WB
• Reactivity: Other
• Dilution range: WB: User Optimized
• Form/Appearance: n/a
• Purity: Biorbyt’ Chemiluminescent Western Blot Kit for GFP combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing GFP and its variants. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains: GFP protein, Mouse IgG control, mouse anti-GFP antibody, anti-mouse IgG peroxidase, Tween-20, BSA, and Femtomax, along with additional supplies and protocol; sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
• Conjugation: Unconjugated
• Storage: See GFP Western Blot kit insert for complete instructions.
• Buffer/Preservatives: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
• Alternative names: GFP Western Blotting Kit, GFP Chemiluminescent Kit
• Note: For research use only
• Application notes: GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of Rockland's optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
• Expiration Date: Please enquire.

Flow Diagram for Chemiluminescent Western Blot Procedure. GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of Biorbyt's optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods.

Western Blot of anti-GFP monoclonal antibody. Lane 1: 64 pg of recombinant GFP protein (p/n orb345957) were spiked into a HeLa cell-derived lysates (p/n orb348668). Lane 2: 32 pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16 pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8 pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4 pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2 pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1 g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0 pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP-conjugated anti-Mouse IgG (p/n orb347544) was performed at a dilution of 1:20000 for 1 h at 4°C. Block: TTBS (p/n orb348540) supplemented with 1% BSA for 1 h at 4°C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.