GFP Tag Antibody

• Catalog Number: orb1927586
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 168AT1211
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Dilution range: IF: 1:25, WB: 1:20000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 27 k
• Target: Purified His-tagged GFP protein was used to produced this monoclonal antibody.
• UniProt ID: C5MKY7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-GFP Tag Antibody at dilution + GFP protein whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela (human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody at a dilution of 1:25 for 1 hour at 37C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody at a dilution of 1: 200 for 60 minutes at 37°C. The nuclear counter stain is DAPI (blue).