GFAP Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1152219
• Category: Antibodies
• Description: GFAP Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GFAP
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1:20000, IHC-P=1:100-1:500, IHC-F=1:100-1:500, IF=1:100-1:500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: GFAP
• UniProt ID: P14136
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Astrocyte; FLJ45472; Glial Fibrillary Acidic Prote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cell line: Mouse primary astrocyte, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152219.

Cell line: Neuro-2a (Negative cell line), Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: No staining on orb1152219.

Paraformaldehyde-fixed, paraffin embedded (MOUSE brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Lane 3: Human U251 cell lysates, Primary: Anti-GFAP (orb1152219) at 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 48 kDa.