GFAP Rabbit Polyclonal Antibody (Cy5)

• Catalog Number: orb913879
• Category: Antibodies
• Description: GFAP Rabbit Polyclonal Antibody (Cy5)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GFAP (51-150/432aa)
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test, IF=1:200-800
• Conjugation: Cy5
• Target: GFAP
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP /cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP /cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat Brian), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP/cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GFAP/cy5 Antibody, conjugated (orb913879) at 1:200 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.