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Catalog Number | orb500829 |
---|---|
Category | Antibodies |
Description | GFAP antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Bovine, Canine, Porcine, Rabbit, Sheep |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human GFAP (51-150/432 aa) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:200-1000, IF=1:200-800, Flow-Cyt=1μg/Test, IHC-F=1:200-1000, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 48 kDa |
Target | GFAP |
UniProt ID | P14136 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of RSC96(blue).Primary Antibody:Rabbit Anti- GFAP antibody; Dilution:1μg in 100 μL 1X PBS containing 0.5% BSA.
Flow cytometry of U87MG.Primary Antibody (green line): Rabbit Anti-GFAP antibody; Dilution:1ug/Test.
ICC analysis of U87MG cell using GFAP polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
IHC-P analysis of human glioma using GFAP Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse brain using GFAP Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of rat brain using GFAP Polyclonal Antibody at 1:200 dilution.
Western blot:Cerebellum (Rat);Cerebellum (Mouse);Eye (Mouse);Primary:Anti-GFAP at 1:300 dilution.
Western blot:U251 Cell;Primary:Anti-GFAP at 1:300 dilution.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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