GFAP Antibody

• Catalog Number: orb1939391
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 183CT3.1.5
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b,k
• Dilution range: IF: 1:10~50, WB: 1:4000, WB: 1:500~1000, IHC-P-Leica: 1:1000, IHC: 1:2000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 49880 Da
• Target: This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
• UniProt ID: P14136
• NCBI: NP_001229305.1, NP_001124491.1, NP_002046.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Glial fibrillary acidic protein, GFAP, GFAP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-GFAP Antibody at 1:4000 dilution. Lane 1: human brain lysate. Lane 2: human cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of GFAP Antibody with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

GFAP Antibody western blot analysis in MCF-7 cell line lysates (35 μg/lane). This demonstrates the GFAP antibody detected the GFAP protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1. diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.