GFAP Antibody

• Catalog Number: orb1294403
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GFAP
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:1000-1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Frozen) 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:100 – 1:300
• Conjugation: Unconjugated
• Target: GFAP
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-GFAP antibody at 1/1000 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose was then blocked for an hour before being incubated with orb1294403 overnight at 4°C.

All lanes: Anti-GFAP antibody at 1/1000 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose was then blocked for an hour before being incubated with orb1294403 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling GFAP antibody with orb1294403 at 1/100.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling GFAP antibody with orb1294403 at 1/100.

Immunofluorescent analysis. Sample: primary cortical neurons; Red: GFAP (orb1294403): 1-200; Blue: DAPI was used as the nuclear counter stain. Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: GFAP (orb1294403): 1-200; Anti-rabbit 488: 1-500.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294403 anti-GFAP antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: GFAP (orb1294403): 1-200; Anti-rabbit 488: 1-500.