GFAP Antibody

• Catalog Number: orb1247969
• Category: Antibodies
• Description: GFAP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Human
• Reactivity: Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-DGEVIKESKQEHKD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa observed in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration:0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GFAP
• UniProt ID: P14136
• NCBI: NP_002046.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GFAP, glial fibrillary acidic protein , FLJ45472 ,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa observed in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration:0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.
• Expiration Date: 12 months from date of receipt.

orb1247969 (5 ug/ml) as the reporter antibody.

orb1247969 (2 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1247969 (0.01 ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

orb1247969 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247969 (0.1 ug/ml) staining of Mouse (A) and (0.01 ug/ml) Rat (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.