GFAP Antibody / Glial Fibrillary Acidic Protein

• Catalog Number: orb606415
• Category: Antibodies
• Description: This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ASTRO/1974R
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: GFAP isolated from pig spinal cord was used as the immunogen for this recombinant GFAP antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant GFAP antibody is available for research use only.
• UniProt ID: P14136
• Storage: Store the recombinant GFAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of frozen human cerebral cortex tissue with recombinant GFAP antibody (blue, clone ASTRO/1974R) and Histone H1 antibody (magenta, clone HH1/957).

IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone ASTRO/1974R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant GFAP antibody (clone ASTRO/1974R) as confirmation of integrity and purity.

Flow cytometry testing of fixed human T98G cells with recombinant GFAP antibody (clone ASTRO/1974R); Red=isotype control, Blue=recombinant GFAP antibody.