GFAP Antibody / Glial Fibrillary Acidic Protein

• Catalog Number: orb1825047
• Category: Antibodies
• Description: GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: GFAP/8616R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the GFAP antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GFAP antibody is available for research use only.
• UniProt ID: P14136
• Storage: Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/8616R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/8616R) as confirmation of integrity and purity.